{"id":6548,"date":"2026-08-03T16:56:14","date_gmt":"2026-08-03T14:56:14","guid":{"rendered":"https:\/\/immunologie-kiel.de\/cyto-ai-powered-analysis\/"},"modified":"2026-08-03T16:56:16","modified_gmt":"2026-08-03T14:56:16","slug":"cyto-ai-powered-analysis","status":"publish","type":"page","link":"https:\/\/immunologie-kiel.de\/en\/cyto-ai-powered-analysis\/","title":{"rendered":"CYTO+ [ AI-Powered Analysis ]"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"6548\" class=\"elementor elementor-6548 elementor-6125\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-4c36155f e-flex e-con-boxed e-con e-parent\" data-id=\"4c36155f\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-5a02c1e2 elementor-widget elementor-widget-text-editor\" data-id=\"5a02c1e2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>CYTO+ is a specialized computational service for spectral flow cytometry analysis that bridges the gap between high-dimensional data generation and biological interpretation. We provide standardized panel design, AI-assisted immunophenotyping, and automated workflows for researchers at CAU and UKSH.<\/p><br><ul>\r\n<li>Automate analysis with AI-driven clustering tools and machine learning.<\/li>\r\n<li>Utilize a validated 43-parameter panel for comprehensive immune profiling.<\/li>\r\n<li>Reduce batch effects via standardized panel design and preprocessing protocols.<\/li>\r\n<li>Access expert support for study planning.<\/li>\r\n<\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-3d0a3d2 e-flex e-con-boxed e-con e-parent\" data-id=\"3d0a3d2\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-fd344e2 cyto-accordion elementor-widget elementor-widget-n-accordion\" data-id=\"fd344e2\" data-element_type=\"widget\" data-e-type=\"widget\" id=\"cyto-accordion\" data-settings=\"{&quot;default_state&quot;:&quot;expanded&quot;,&quot;max_items_expended&quot;:&quot;one&quot;,&quot;n_accordion_animation_duration&quot;:{&quot;unit&quot;:&quot;ms&quot;,&quot;size&quot;:400,&quot;sizes&quot;:[]}}\" data-widget_type=\"nested-accordion.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"e-n-accordion\" aria-label=\"Accordion. Open links with Enter or Space, close with Escape, and navigate with Arrow Keys\">\n\t\t\t\t\t\t<details id=\"e-n-accordion-item-2650\" class=\"e-n-accordion-item\" open>\n\t\t\t\t<summary class=\"e-n-accordion-item-title\" data-accordion-index=\"1\" tabindex=\"0\" aria-expanded=\"true\" aria-controls=\"e-n-accordion-item-2650\" >\n\t\t\t\t\t<span class='e-n-accordion-item-title-header'><div class=\"e-n-accordion-item-title-text\"> Why Choose CYTO+? <\/div><\/span>\n\t\t\t\t\t\t\t<span class='e-n-accordion-item-title-icon'>\n\t\t\t<span class='e-opened' ><i aria-hidden=\"true\" class=\"fas fa-minus\"><\/i><\/span>\n\t\t\t<span class='e-closed'><i aria-hidden=\"true\" class=\"fas fa-plus\"><\/i><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t<div role=\"region\" aria-labelledby=\"e-n-accordion-item-2650\" class=\"elementor-element elementor-element-82318fe e-con-full e-flex e-con e-child\" data-id=\"82318fe\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-6392b07 elementor-widget elementor-widget-text-editor\" data-id=\"6392b07\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul style=\"margin-top: 0cm;\" type=\"disc\">\n<li class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: 150%; mso-list: l0 level1 lfo1; tab-stops: list 36.0pt;\"><b><span lang=\"EN-GB\">Comprehensive workflow integration:<\/span><\/b><span lang=\"EN-GB\">\u00a0From antibody selection to publication-ready dashboards, CYTO+ provides end-to-end support for spectral cytometry projects.<\/span><\/li>\n<li class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: 150%; mso-list: l0 level1 lfo1; tab-stops: list 36.0pt;\"><b><span lang=\"EN-GB\">Standardized protocols:<\/span><\/b><span lang=\"EN-GB\">\u00a0Optimized SOPs for sample biobanking, staining, and clinical standardization ensure reproducible, high-quality data across batches.<\/span><\/li>\n<li class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: 150%; mso-list: l0 level1 lfo1; tab-stops: list 36.0pt;\"><b><span lang=\"EN-GB\">AI-powered analysis:<\/span><\/b><span lang=\"EN-GB\">\u00a0Automated clustering, UMAP visualization, and LLM-assisted cell population annotation eliminate manual gating bottlenecks.<\/span><\/li>\n<li class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: 150%; mso-list: l0 level1 lfo1; tab-stops: list 36.0pt;\"><b><span lang=\"EN-GB\">Reduced batch effects:<\/span><\/b><span lang=\"EN-GB\">\u00a0Unified panel design and automated preprocessing minimize technical variability in patient cohorts.<\/span><\/li>\n<li class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: 150%; mso-list: l0 level1 lfo1; tab-stops: list 36.0pt;\"><b><span lang=\"EN-GB\">Expert support:<\/span><\/b><span lang=\"EN-GB\">\u00a0Led by Dr. Henry Nording with dedicated AI-cytometry technical support from Jacob von Esebeck.<\/span><\/li>\n<\/ul>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t<details id=\"e-n-accordion-item-2651\" class=\"e-n-accordion-item\" >\n\t\t\t\t<summary class=\"e-n-accordion-item-title\" data-accordion-index=\"2\" tabindex=\"-1\" aria-expanded=\"false\" aria-controls=\"e-n-accordion-item-2651\" >\n\t\t\t\t\t<span class='e-n-accordion-item-title-header'><div class=\"e-n-accordion-item-title-text\"> The CYTO+ Panel: 37+6 Spectral OMIP <\/div><\/span>\n\t\t\t\t\t\t\t<span class='e-n-accordion-item-title-icon'>\n\t\t\t<span class='e-opened' ><i aria-hidden=\"true\" class=\"fas fa-minus\"><\/i><\/span>\n\t\t\t<span class='e-closed'><i aria-hidden=\"true\" class=\"fas fa-plus\"><\/i><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t<div role=\"region\" aria-labelledby=\"e-n-accordion-item-2651\" class=\"elementor-element elementor-element-65aae84 e-con-full e-flex e-con e-child\" data-id=\"65aae84\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-0e1e441 elementor-widget elementor-widget-text-editor\" data-id=\"0e1e441\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"margin-bottom: 0cm; line-height: 150%;\"><span lang=\"EN-GB\">CYTO+ employs a validated 43-parameter spectral flow cytometry panel optimized for 5-laser systems (Cytek Aurora), balancing comprehensive immune coverage with experimental flexibility.<\/span><\/p>\n<p style=\"margin-bottom: 0cm; line-height: 150%;\"><b>Panel architecture:<\/b><\/p>\n<ul style=\"margin-top: 0cm;\" type=\"disc\">\n<li style=\"margin-bottom: 0cm; line-height: 150%; mso-list: l0 level1 lfo1; tab-stops: list 36.0pt;\"><b><span lang=\"EN-GB\">37-marker backbone:<\/span><\/b><span lang=\"EN-GB\">\u00a0Covers all major leukocyte populations in human peripheral blood with resolution comparable to<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32830910\/\"><span style=\"font-variant-numeric: normal; font-variant-east-asian: normal; font-variant-caps: small-caps; font-variant-alternates: normal; font-variant-position: normal; font-variant-emoji: normal;\"> OMIP-069<\/span><\/a><\/span>\n<ul style=\"margin-top: 0cm;\" type=\"circle\">\n<li style=\"margin-bottom: 0cm; line-height: 150%; mso-list: l0 level2 lfo1; tab-stops: list 72.0pt;\"><span lang=\"EN-GB\">T cells: Na\u00efve, central\/effector memory (TCM\/TEM), TEMRA, regulatory T cells (na\u00efve\/activated Tregs), MAIT cells, and Th1\/Th2 subsets via chemokine receptor profiling.<\/span><\/li>\n<li style=\"margin-bottom: 0cm; line-height: 150%; mso-list: l0 level2 lfo1; tab-stops: list 72.0pt;\"><span lang=\"EN-GB\">Myeloid cells: Monocytes (classical\/intermediate\/non-classical), dendritic cells (cDC1, cDC2, pDC), and basophils.<\/span><\/li>\n<li style=\"margin-bottom: 0cm; line-height: 150%; mso-list: l0 level2 lfo1; tab-stops: list 72.0pt;\"><span lang=\"EN-GB\">Lymphocytes: B cells (na\u00efve, marginal zone-like, memory, plasmablasts), NK cells (CD56dim\/bright), innate lymphoid cells (ILCs).<\/span><\/li>\n<li style=\"margin-bottom: 0cm; line-height: 150%; mso-list: l0 level2 lfo1; tab-stops: list 72.0pt;\"><span lang=\"EN-GB\">Rare populations: Circulating endothelial cells (CD45<\/span><span lang=\"EN-GB\" style=\"font-family: 'Cambria Math',serif; mso-bidi-font-family: 'Cambria Math'; mso-ansi-language: EN-GB;\">\u207b<\/span><span lang=\"EN-GB\">CD31<\/span><span lang=\"EN-GB\" style=\"font-family: 'Cambria Math',serif; mso-bidi-font-family: 'Cambria Math'; mso-ansi-language: EN-GB;\">\u207a<\/span><span lang=\"EN-GB\">).<\/span><\/li>\n<\/ul>\n<\/li>\n<li style=\"margin-bottom: 0cm; line-height: 150%; mso-list: l0 level1 lfo1; tab-stops: list 36.0pt;\"><b><span lang=\"EN-GB\">6 flexible drop-in channels:<\/span><\/b><span lang=\"EN-GB\">\u00a0Pre-allocated slots for disease- or context-specific markers (e.g., checkpoint inhibitors PD-1, TIGIT; activation markers CD69, CD152; tissue-specific antigens) without disrupting backbone resolution.<\/span><\/li>\n<li style=\"margin-bottom: 0cm; line-height: 150%; mso-list: l0 level1 lfo1; tab-stops: list 36.0pt;\"><b><span lang=\"EN-GB\">Low complexity index:<\/span><\/b><span lang=\"EN-GB\">\u00a026.66 (vs. 53.72 for OMIP-069), enabling robust spectral unmixing with minimal interference.<\/span><\/li>\n<\/ul>\n<p style=\"margin-bottom: 0cm; line-height: 150%;\"><b><span lang=\"EN-GB\">Sample compatibility:<\/span><\/b><span lang=\"EN-GB\"><br \/>Validated for cryopreserved PBMCs, RBC-lysed whole blood, and PROT1-stabilized samples.<\/span><\/p>\n<div>\n<div>\n<div>\n<p>\u00a0<\/p>\n<\/div>\n<\/div>\n<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t<details id=\"e-n-accordion-item-2652\" class=\"e-n-accordion-item\" >\n\t\t\t\t<summary class=\"e-n-accordion-item-title\" data-accordion-index=\"3\" tabindex=\"-1\" aria-expanded=\"false\" aria-controls=\"e-n-accordion-item-2652\" >\n\t\t\t\t\t<span class='e-n-accordion-item-title-header'><div class=\"e-n-accordion-item-title-text\"> Service Packages <\/div><\/span>\n\t\t\t\t\t\t\t<span class='e-n-accordion-item-title-icon'>\n\t\t\t<span class='e-opened' ><i aria-hidden=\"true\" class=\"fas fa-minus\"><\/i><\/span>\n\t\t\t<span class='e-closed'><i aria-hidden=\"true\" class=\"fas fa-plus\"><\/i><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t<div role=\"region\" aria-labelledby=\"e-n-accordion-item-2652\" class=\"elementor-element elementor-element-3cb3f05 e-con-full e-flex e-con e-child\" data-id=\"3cb3f05\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ab57f63 elementor-widget elementor-widget-text-editor\" data-id=\"ab57f63\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: 150%;\"><span lang=\"EN-GB\">CYTO+ offers modular service tiers to support projects from initial planning through publication.<\/span><\/p>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a2f6971 cyto-plus-table elementor-widget elementor-widget-text-editor\" data-id=\"a2f6971\" data-element_type=\"widget\" data-e-type=\"widget\" id=\"cyto-plus-table\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<table>\n<thead>\n<tr>\n<td>\n<p><strong>Package<\/strong><\/p>\n<p>\u00a0<\/p>\n<\/td>\n<td>\n<p><strong>Description<\/strong><\/p>\n<p>\u00a0<\/p>\n<\/td>\n<td>\n<p><strong>Fee<\/strong><\/p>\n<p>\u00a0<\/p>\n<\/td>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>\n<p><strong>Starter<\/strong><\/p>\n<\/td>\n<td>\n<p>Panel feasibility check and up to 3 hours of design coaching, including evaluation of fluorochrome-marker combinations and antibody titration strategies.<\/p>\n<\/td>\n<td>\n<p>90 \u20ac<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<p><strong>Study Planning<\/strong><\/p>\n<\/td>\n<td>\n<p>Guidance on sample collection, RBC lysis\/fixation protocols, quality-control run, and pilot measurement (two half-day sessions).<\/p>\n<\/td>\n<td>\n<p>300 \u20ac<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<p><strong>Deep-Insight<\/strong><\/p>\n<\/td>\n<td>\n<p>Machine learning cluster analysis with interactive heatmaps and dashboards; includes user onboarding to the analysis pipeline (two half-day sessions).<\/p>\n<\/td>\n<td>\n<p>300 \u20ac<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<p><strong>Academy <\/strong>(scheduled when minimum enrollment is reached)<\/p>\n<\/td>\n<td>\n<p>Intensive two-day hands-on coding workshop covering spectral unmixing, R-based analysis pipeline operation, and custom workflow adaptation. Scheduled when minimum enrollment is reached (typically 6\u20138 participants). Includes course materials, sample datasets, and certificate of completion. To express interest or inquire about the next scheduled session, please contact Jacob von Esebeck (Jacob.vonEsebeck@uksh.de). Participants should have basic familiarity with R programming; introductory materials will be provided upon registration.<\/p>\n<\/td>\n<td>\n<p>TBA*<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t<details id=\"e-n-accordion-item-2653\" class=\"e-n-accordion-item\" >\n\t\t\t\t<summary class=\"e-n-accordion-item-title\" data-accordion-index=\"4\" tabindex=\"-1\" aria-expanded=\"false\" aria-controls=\"e-n-accordion-item-2653\" >\n\t\t\t\t\t<span class='e-n-accordion-item-title-header'><div class=\"e-n-accordion-item-title-text\"> How to Use CYTO+ <\/div><\/span>\n\t\t\t\t\t\t\t<span class='e-n-accordion-item-title-icon'>\n\t\t\t<span class='e-opened' ><i aria-hidden=\"true\" class=\"fas fa-minus\"><\/i><\/span>\n\t\t\t<span class='e-closed'><i aria-hidden=\"true\" class=\"fas fa-plus\"><\/i><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t<div role=\"region\" aria-labelledby=\"e-n-accordion-item-2653\" class=\"elementor-element elementor-element-f936b6a e-flex e-con-boxed e-con e-child\" data-id=\"f936b6a\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-824ec90 elementor-widget elementor-widget-text-editor\" data-id=\"824ec90\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Step 1: Initial consultation<\/strong><br \/>Contact the CYTO+ team via email or phone to discuss your research question. CYTO+ provides flexible entry points depending on your project stage and analysis needs. Whether you have existing high-dimensional data requiring expert analysis, are planning a new spectral cytometry experiment, or wish to develop in-house analysis capabilities, CYTO+ offers tailored support pathways.<\/p>\n<p><strong>Find your starting point:<\/strong><\/p>\n<ul>\n<li><strong>Existing data analysis:<\/strong>\u00a0You have spectral flow cytometry data and need AI-assisted clustering, annotation, and interpretation \u2192\u00a0<strong>Deep-Insight<\/strong>\u00a0package.<\/li>\n<li><strong>Experiment planning:<\/strong>\u00a0You\u2019re designing a new study and need panel design guidance or marker selection support \u2192\u00a0<strong>Starter<\/strong>\u00a0consultation.<\/li>\n<li><strong>Complete study support:<\/strong>\u00a0You need end-to-end assistance from experimental design through data analysis for clinical or longitudinal studies \u2192 combine\u00a0<strong>Study Planning<\/strong>\u00a0+\u00a0<strong>Deep-Insight<\/strong>.<\/li>\n<li><strong>Learn to analyze independently:<\/strong>\u00a0You want hands-on training in R-based workflows and the CYTO+ pipeline \u2192\u00a0<strong>Academy<\/strong>\u00a0workshop (scheduled on demand).<\/li>\n<li><strong>Custom support:<\/strong>\u00a0Need individual pipeline assistance, troubleshooting, or specialized analysis? We offer personalized consultation beyond standard packages.<\/li>\n<\/ul>\n<p>\u00a0<\/p>\n<p>\u00a0<\/p>\n<p><strong>Step 2: Computational support <\/strong><\/p>\n<p>CYTO+ operates like a flow cytometry core facility\u2014only the \u201cinstrument time\u201d is compute time and expert guidance for\u00a0<strong>high\u2011dimensional flow cytometry analysis<\/strong>. Depending on the dataset size and your preferred working style, analysis can run on CYTO+ workstations (remote access) or on the CAU HPC cluster for large datasets with high event counts.<\/p>\n<ul>\n<li><strong>CYTO+ Analysis Workstations<\/strong><\/li>\n<\/ul>\n<ul>\n<li>Operation mode:\u00a0Autonomous usage (remote access) or assisted analysis sessions<\/li>\n<li>Specifications:\u00a0High-performance workstations optimized for spectral flow cytometry data<\/li>\n<li>Capacity:\u00a0Suitable for small to medium-sized datasets (up to ~20\u201330 million total events after cleaning)<\/li>\n<li>Booking:\u00a0Reservable via the online booking system under \u201cCytometry Equipment\u201d<\/li>\n<li>Features:\u00a0Pre-installed CYTO+ R-pipeline environment, FlowJo, and SpectroFlo for post-acquisition analysis<\/li>\n<li>For larger datasets: CYTO+ supports execution on the CAU HPC cluster (including cluster-ready workflows).<\/li>\n<\/ul>\n<ul>\n<li><strong>Support model (per hour)<\/strong>\n<ul>\n<li>Full service: CYTO+ runs the pipeline for you and delivers curated results and dashboards.<\/li>\n<li>Assisted usage: CYTO+ works with you\u00a0<em>per hour<\/em>\u00a0while teaching you how to run the established workflows (best suited for users who can read and adapt R code, without writing everything from scratch).<\/li>\n<\/ul>\n<\/li>\n<li><strong>What the pipeline covers (end-to-end)<\/strong>\n<ul>\n<li>Preprocessing &amp; QC: per-file debris gating, fixed compensation handling, debris\/doublet\/dead-cell exclusion using a \u201cmolecular ruler\u201d, robust logicle transformation, gaussNorm (or KDE-based) normalization, trimming, and batch-effect detection visualizations.<\/li>\n<li>Clustering &amp; curation: FlowSOM clustering, clustering-depth assessment, UMAP, metadata overlays (e.g., hexbins), and systematic artifact detection\/curation (dominated clusters, very small clusters, mixed-lineage clusters, mutually exclusive clusters driven by residual batch effects).<\/li>\n<li>Annotation &amp; readouts: AI-assisted cluster naming and downstream feature\/readout extraction designed for real-world patient datasets and trait correlations.<\/li>\n<\/ul>\n<\/li>\n<li><strong>UI-first exploration (optional)<\/strong>\n<ul>\n<li>Shiny-based dashboards (e.g., ShinyCell2) enable interactive exploration of processed data without day-to-day coding, while keeping the full analysis reproducible and scriptable.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>\u00a0<\/p>\n<p><strong>Step 3: Ongoing collaboration<\/strong><br \/>CYTO+ supports iterative refinement of analyses, follow-up experiments, and multi-omics integration (e.g., linking flow cytometry phenotypes with transcriptomic or proteomic data via the university\u2019s Bioinformatics Platform). Schedule additional consultations as needed to discuss findings, troubleshoot technical challenges, or plan publication strategies.<\/p>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t<details id=\"e-n-accordion-item-2654\" class=\"e-n-accordion-item\" >\n\t\t\t\t<summary class=\"e-n-accordion-item-title\" data-accordion-index=\"5\" tabindex=\"-1\" aria-expanded=\"false\" aria-controls=\"e-n-accordion-item-2654\" >\n\t\t\t\t\t<span class='e-n-accordion-item-title-header'><div class=\"e-n-accordion-item-title-text\"> Getting Started <\/div><\/span>\n\t\t\t\t\t\t\t<span class='e-n-accordion-item-title-icon'>\n\t\t\t<span class='e-opened' ><i aria-hidden=\"true\" class=\"fas fa-minus\"><\/i><\/span>\n\t\t\t<span class='e-closed'><i aria-hidden=\"true\" class=\"fas fa-plus\"><\/i><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t<div role=\"region\" aria-labelledby=\"e-n-accordion-item-2654\" class=\"elementor-element elementor-element-bf7aa20 e-flex e-con-boxed e-con e-child\" data-id=\"bf7aa20\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-f2eaf7c elementor-widget elementor-widget-text-editor\" data-id=\"f2eaf7c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: 150%;\"><span lang=\"EN-GB\">Contact the CYTO+ team to schedule an initial consultation (no charge) to discuss your project and identify the optimal service pathway:<\/span><\/p>\n<p>\u00a0<\/p>\n<ul style=\"margin-top: 0cm;\" type=\"disc\">\n<li class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: 150%; mso-list: l0 level1 lfo1; tab-stops: list 36.0pt;\"><b><span lang=\"EN-GB\">CYTO+ Responsible Scientist:<\/span><\/b><span lang=\"EN-GB\">\u00a0Dr. med. Henry Nording (<\/span><a href=\"mailto:Henry.Nording@uksh.de\"><span lang=\"EN-GB\">Henry.Nording@uksh.de<\/span><\/a><span lang=\"EN-GB\">)<\/span><\/li>\n<li class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: 150%; mso-list: l0 level1 lfo1; tab-stops: list 36.0pt;\"><b><span lang=\"EN-GB\">AI-Cytometry Technician:<\/span><\/b><span lang=\"EN-GB\">\u00a0Jacob von Esebeck (<\/span><a href=\"mailto:Jacob.vonEsebeck@uksh.de\"><span lang=\"EN-GB\">Jacob.vonEsebeck@uksh.de<\/span><\/a><span lang=\"EN-GB\">)<\/span><\/li>\n<li class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: 150%; mso-list: l0 level1 lfo1; tab-stops: list 36.0pt;\"><b>General enquiries:<\/b>\u00a0<a href=\"mailto:cytometry.corefacility@uksh.de\">cytometry.corefacility@uksh.de<\/a><\/li>\n<\/ul>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t<details id=\"e-n-accordion-item-2655\" class=\"e-n-accordion-item\" >\n\t\t\t\t<summary class=\"e-n-accordion-item-title\" data-accordion-index=\"6\" tabindex=\"-1\" aria-expanded=\"false\" aria-controls=\"e-n-accordion-item-2655\" >\n\t\t\t\t\t<span class='e-n-accordion-item-title-header'><div class=\"e-n-accordion-item-title-text\"> FAQs: Frequently Asked Questions <\/div><\/span>\n\t\t\t\t\t\t\t<span class='e-n-accordion-item-title-icon'>\n\t\t\t<span class='e-opened' ><i aria-hidden=\"true\" class=\"fas fa-minus\"><\/i><\/span>\n\t\t\t<span class='e-closed'><i aria-hidden=\"true\" class=\"fas fa-plus\"><\/i><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t<div role=\"region\" aria-labelledby=\"e-n-accordion-item-2655\" class=\"elementor-element elementor-element-763a00d e-flex e-con-boxed e-con e-child\" data-id=\"763a00d\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-4513ef5 elementor-widget elementor-widget-text-editor\" data-id=\"4513ef5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: 150%;\"><b><span lang=\"EN-GB\">Do I need to use the standard 37+6 panel?<\/span><\/b><span lang=\"EN-GB\"><br>No. While the 37+6 backbone is validated for comprehensive immune monitoring, we fully support&nbsp;<b>custom panel design<\/b>. You can modify the six drop-in channels or collaborate with us to build a completely bespoke high-dimensional panel tailored to your specific research question.<\/span><\/p><p class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: 150%;\"><span lang=\"EN-GB\"><br><\/span><\/p><br><p class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: 150%;\"><b><span lang=\"EN-GB\">Can I analyze my own external datasets with CYTO+?<\/span><\/b><span lang=\"EN-GB\"><br>Yes. We accept high-dimensional spectral flow cytometry data from any source (e.g., external core facilities or public repositories). Our&nbsp;<b>Deep-Insight<\/b>&nbsp;package is designed to process and normalize external datasets, provided they meet basic quality standards (which we assess during the initial consultation).<\/span><\/p><p class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: 150%;\"><span lang=\"EN-GB\"><br><\/span><\/p><br><p class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: 150%;\"><b><span lang=\"EN-GB\">Is CYTO+ suitable for clinical multi-center studies?<\/span><\/b><span lang=\"EN-GB\"><br>Absolutely. Our pipeline is optimized for&nbsp;<b>clinical cytometry<\/b>&nbsp;cohorts. We provide standardized SOPs, batch-normalization strategies (gaussNorm), and robust quality control protocols specifically designed to minimize site-to-site variability and batch effects in longitudinal patient studies.<\/span><\/p><p class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: 150%;\"><span lang=\"EN-GB\"><br><\/span><\/p><br><p class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: 150%;\"><b><span lang=\"EN-GB\">How is data security handled?<\/span><\/b><span lang=\"EN-GB\"><br>Patient-related analyses are performed within the firewall-protected UKSH clinical network to keep identifiable information inside the hospital IT environment. For compute-intensive runs on CAU high-performance resources and the German National High-Performance Computing network (NHR), only fully anonymized research datasets are used, in line with NHR data protection rules. NHR explicitly states that storing or processing research data containing personal information on NHR systems is prohibited and recommends working with anonymized data.<\/span><\/p><br><p><\/p><br><p class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: 150%;\"><b><span lang=\"EN-GB\">How does CYTO+ differ from the standard Cytometry Facility?<\/span><\/b><span lang=\"EN-GB\"><br>The standard CYTO facility focuses on&nbsp;<b>instrument operation<\/b>&nbsp;and cell sorting. CYTO+ operates as a&nbsp;<b>computational core<\/b>, focusing exclusively on what happens&nbsp;<i>before<\/i>&nbsp;(panel design, sample prep) and&nbsp;<i>after<\/i>&nbsp;acquisition (spectral unmixing, AI-driven clustering, biological interpretation).<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-fc5ce1f elementor-widget elementor-widget-html\" data-id=\"fc5ce1f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"html.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<script>\r\ndocument.addEventListener(\"DOMContentLoaded\", function () {\r\n\r\n    const target = document.querySelector('#cyto-accordion, .cyto-accordion');\r\n    if (!target) return;\r\n\r\n    \/\/ Beobachter, der sofort reagiert, wenn Elementor das Accordion rendert\r\n    const observer = new MutationObserver(() => {\r\n        const firstItem = target.querySelector('details.e-n-accordion-item');\r\n        if (firstItem) {\r\n            firstItem.removeAttribute('open'); \/\/ verhindert das automatische \u00d6ffnen\r\n        }\r\n    });\r\n\r\n    observer.observe(target, { childList: true, subtree: true });\r\n\r\n    \/\/ Sicherheitshalber nach 200ms nochmal schlie\u00dfen\r\n    setTimeout(() => {\r\n        const firstItem = target.querySelector('details.e-n-accordion-item');\r\n        if (firstItem) firstItem.removeAttribute('open');\r\n    }, 200);\r\n});\r\n<\/script>\r\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>CYTO+ is a specialized computational service for spectral flow cytometry analysis that bridges the gap between high-dimensional data generation and biological interpretation. We provide standardized panel design, AI-assisted immunophenotyping, and automated workflows for researchers at CAU and UKSH. Automate analysis with AI-driven clustering tools and machine learning. Utilize a validated 43-parameter panel for comprehensive immune [&hellip;]<\/p>\n","protected":false},"author":10,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":""},"class_list":["post-6548","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/immunologie-kiel.de\/en\/wp-json\/wp\/v2\/pages\/6548","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/immunologie-kiel.de\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/immunologie-kiel.de\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/immunologie-kiel.de\/en\/wp-json\/wp\/v2\/users\/10"}],"replies":[{"embeddable":true,"href":"https:\/\/immunologie-kiel.de\/en\/wp-json\/wp\/v2\/comments?post=6548"}],"version-history":[{"count":3,"href":"https:\/\/immunologie-kiel.de\/en\/wp-json\/wp\/v2\/pages\/6548\/revisions"}],"predecessor-version":[{"id":6554,"href":"https:\/\/immunologie-kiel.de\/en\/wp-json\/wp\/v2\/pages\/6548\/revisions\/6554"}],"wp:attachment":[{"href":"https:\/\/immunologie-kiel.de\/en\/wp-json\/wp\/v2\/media?parent=6548"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}