{"id":6555,"date":"2026-08-03T16:57:06","date_gmt":"2026-08-03T14:57:06","guid":{"rendered":"https:\/\/immunologie-kiel.de\/cytometry-news\/"},"modified":"2026-08-03T16:58:32","modified_gmt":"2026-08-03T14:58:32","slug":"cytometry-news","status":"publish","type":"page","link":"https:\/\/immunologie-kiel.de\/en\/cytometry-news\/","title":{"rendered":"Cytometry – News"},"content":{"rendered":"\t\t
\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t

Cytometry Dates<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t
\t\t
\n\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t
FACS Club<\/div><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t
\n
\"\"<\/figure>
\n

<\/p>\n<\/div><\/div>\n\n\n\n

<\/p>\n\n\n\n

Presenter:
\nLaura Bauer (AG Hutloff)<\/strong><\/p>\n\n\n\n

Titel:
High-dimensional spectral flow cytometry – using FlowJo plugins for multi-parameter analysis<\/strong><\/strong><\/p>\n\n\n\n

Abstract:
Spectral flow cytometry is a technological advancement that provides us researchers with the opportunity to measure a multitude of markers at a previously unattainable level of detail. The development of complex flow panels has led to a significant expansion in data dimensionality. The integration of FlowJo plugins facilitates not only the effective preprocessing of data but also enables a deeper characterisation of sample populations. Implementation of dimension reduction, clustering, and visualisation tools has the potential to support the identification of novel populations and coexpression patterns.<\/em><\/a><\/p>\n\n\n\n

In this presentation, Laura will provide a brief overview of a potential high-dimensional FlowJo analysis workflow, along with a live demo of some of the steps involved.<\/em><\/em><\/p>\n\n\n\n

<\/p>\n<\/div><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

\n\t\t\t\t
\n\t\t\t\t\t
18.09.2026 - 09:30\n\n\n\n<\/div><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<\/div><\/div><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
\n\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t

Cytometry Publications<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t\t\t

Here you can find a list of publications that were created in collaboration with or using the equipment of the Flow Cytometry Core Facility.\u00a0<\/p>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

\n\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\n\t\t\t\t\t
2025 <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/i><\/span>\n\t\t\t<\/i><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t\t\t
    \n
  1. H\u00fcttermann, Leonard; Schr\u00f6der, Lena C.; Shetty, Prithviraj M. V.; Jonker, Timo; Hille, Susanne S.; Kliesow Remes, Anca et al. (2025): Directed Evolution of AAV9 for Efficient Gene Expression in Cardiomyocytes In Vitro and In Vivo. In: Human gene therapy <\/em>36 (3-4), S. 101\u2013115. DOI: 10.1089\/hum.2024.126.<\/li>\n
  2. Schacht, Sarah-Sophie; Graffunder, Josefine; Durek, Pawel; Wehrenberg, Jonas; Siracusa, Annette; Biese, Charlotte et al. (2025): Activation and maturation of antigen-specific B cells in nonectopic lung infiltrates are independent of germinal center reactions in the draining lymph node. In: Cellular & molecular immunology <\/em>22 (6), S. 612\u2013627. DOI: 10.1038\/s41423-025-01285-8.<\/li>\n
  3. Seifert, Nicole; Reinke, Sarah; Grund, Johanna; M\u00fcller-Meinhard, Berit; Richter, Julia; Heilmann, Thorsten et al. (2025): T-cell diversity and exclusion of blood-derived T-cells in the tumor microenvironment of classical Hodgkin Lymphoma. In: Leukemia <\/em>39 (3), S. 684\u2013693. DOI: 10.1038\/s41375-024-02490-6.<\/li>\n<\/ol>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t
    \n\t\t\t\t\n\t\t\t\t\t
    2024 <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/i><\/span>\n\t\t\t<\/i><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
    \n\t\t\t\t
    \n\t\t\t\t
    \n\t\t\t\t\t\t\t\t\t
      \n
    1. Bastian, Lorenz; Beder, Thomas; Barz, Malwine J.; Bendig, Sonja; Bartsch, Lorenz; Walter, Wencke et al. (2024): Developmental trajectories and cooperating genomic events define molecular subtypes of BCR::ABL1-positive ALL. In: Blood <\/em>143 (14), S. 1391\u20131398. DOI: 10.1182\/blood.2023021752.<\/li>\n
    2. H\u00fcttermann, Leonard; Schr\u00f6der, Lena C.; Shetty, Prithviraj M. V.; Jonker, Timo; Hille, Susanne S.; Kliesow Remes, Anca et al. (2025): Directed Evolution of AAV9 for Efficient Gene Expression in Cardiomyocytes In Vitro and In Vivo. In: Human gene therapy <\/em>36 (3-4), S. 101\u2013115. DOI: 10.1089\/hum.2024.126.<\/li>\n
    3. Klapper, Wolfram; Seifert, Nicole; Reinke, Sarah; Grund, Johanna; M\u00fcller-Meinhard, Berit; Richter, Julia et al. (2024): T-cell diversity and exclusion of blood-derived T-cells in the tumor microenvironment of classical Hodgkin Lymphoma.<\/li>\n
    4. Saggau, Carina; Bacher, Petra; Esser, Daniela; Rasa, Mahdi; Meise, Silja; Mohr, Nicola et al. (2024): Autoantigen-specific CD4+ T cells acquire an exhausted phenotype and persist in human antigen-specific autoimmune diseases. In: Immunity <\/em>57 (10), 2416-2432.e8. DOI: 10.1016\/j.immuni.2024.08.005.<\/li>\n
    5. Schewe, Denis M.; Vogiatzi, Fotini; M\u00fcnnich, Ira A.; Zeller, Tobias; Windisch, Roland; Wichmann, Christian et al. (2024): Enhanced potency of immunotherapy against B-cell precursor acute lymphoblastic leukemia by combination of an Fc-engineered CD19 antibody and CD47 blockade. In: HemaSphere <\/em>8 (2), e48. DOI: 10.1002\/hem3.48.<\/li>\n
    6. Schultze, Joachim; Agrawal, Shobhit; Scholz, Rebekka; Hartmann, Celia; Hetzel, Leon; Carraro, Caterina et al. (2024): Community effort to unravel the complex human neuronal landscape exemplified for the striatum.<\/li>\n<\/ol>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t
      \n\t\t\t\t\n\t\t\t\t\t
      2023 <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/i><\/span>\n\t\t\t<\/i><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
      \n\t\t\t\t
      \n\t\t\t\t
      \n\t\t\t\t\t\t\t\t\t
        \n
      1. Beder, Thomas; Hansen, Bj\u00f6rn-Thore; Hartmann, Alina M.; Zimmermann, Johannes; Amelunxen, Eric; Wolgast, Nadine et al. (2023): The Gene Expression Classifier ALLCatchR Identifies B-cell Precursor ALL Subtypes and Underlying Developmental Trajectories Across Age. In: HemaSphere <\/em>7 (9), e939. DOI: 10.1097\/HS9.0000000000000939.<\/li>\n
      2. Martini, Gabriela Rios; Tikhonova, Ekaterina; Rosati, Elisa; DeCelie, Meghan Bialt; Sievers, Laura Katharina; Tran, Florian et al. (2023): Selection of cross-reactive T cells by commensal and food-derived yeasts drives cytotoxic TH1 cell responses in Crohn’s disease. In: Nature medicine <\/em>29 (10), S. 2602\u20132614. DOI: 10.1038\/s41591-023-02556-5.<\/li>\n
      3. Schwarz, Carsten; Eschenhagen, Patience; Schmidt, Henrijette; Hohnstein, Thordis; Iwert, Christina; Grehn, Claudia et al. (2023): Antigen specificity and cross-reactivity drive functionally diverse anti-Aspergillus fumigatus T cell responses in cystic fibrosis. In: The Journal of clinical investigation <\/em>133 (5). DOI: 10.1172\/JCI161593.<\/li>\n<\/ol>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
        \n\t\t\t\t
        \n\t\t\t\t\t