Panel design
We support your study with expert flow cytometry panel design. Whether you need a completely custom setup or an adaptation of established panels, we find the right solution for your research question.
Available Flow Cytometry Panels
For straightforward measurements, we can offer in-house validated panels or validated published standard panels.
T cell and innate Subset phenotyping
Panel focus: | Lymphocyte immunophenotyping with focus on T cells and small innate and innate-like populations |
Populations markers | ||
CD1c | Dendritic cells | |
CD3 | T cells | |
TCR V alpha 7.2 | MAIT cells (invariant abTCR) | |
TCRVdelta1 | T cells (Vd1 TCR) | |
TCRVdelta2 | T cells (Vd1 TCR) | |
TCRgamma/delta | T cells (pan-gd TCR) | |
CD4 | CD4+ T helper cells | |
CD8 | CD8+ Cytotoxic T cells | |
CD14 | Monocytes | |
CD16 (FcγRIII) | NK cells, monocytes, macrophages, neutrophil granulocytes, certain T cells | |
CD19 | B cells | |
CD33 | Myeloid linage | |
CD45 | Leukocytes | |
CD56 | NK and NK T cells | |
CD64 | Monocytes, Macrophages | |
Differentiation- /Subpopulation markers | ||
CD25 | Activated T cells and regulatory cells (Treg) | |
CD27 | Central memory- & naïve T cells | |
CD45RA | Terminal differentiated- & naive T cells | |
CD117 (cKit) | ||
CD127 | IL7Ra (low expression on Treg) | |
CD197 (CCR7) | Naive T cells | |
CD294 CRTH-2 | ||
Activation markers | ||
CD38 | ||
HLA-DR | MHC class II molecule | |
Co-stimulatory and Inhibitory Receptors | ||
CD159a (NKG2A) | ||
CD314 (NKG2D) | ||
CD337 (NKp30) | ||
CD335 (NKp46) | ||
CD161 (KLRG1) | ||
Live/Dead | ||
Live/Dead | Allows identification of dead cells |
T- & NK cell activation and phenotyping
Panel focus:
| Identification of subpopulations and exhaustion status, and ex vivo activation marker upregulation |
Populations markers | ||
CD3 | T cells | |
TCRVdelta1 | T cells (Vd1 TCR) | |
TCRVdelta2 | T cells (Vd1 TCR) | |
TCRgamma/delta | T cells (pan-gd TCR) | |
CD4 | CD4+ T helper cells | |
CD8 | CD8+ Cytotoxic T cells | |
CD16 (FcγRIII) | NK cells, monocytes, macrophages, neutrophil granulocytes, certain T cells | |
CD56 | NK and NK T cells | |
Differentiation- /Subpopulation markers | ||
CCR7 | Naive T cells | |
CD27 | Central memory- & naïve T cells | |
CD45RA | Terminal differentiated- & naive T cells | |
CD25 | Activated T cells and regulatory cells (Treg) | |
CD127 | IL7R (low expression on Treg) | |
FoxP3 | Treg cells | |
Activation markers | ||
CD25 | Activated T cells and regulatory cells (Treg) | |
CD38 | ||
CD69 | ||
CD137 | ||
CD154 (CD40L) | ||
HLA-DR | ||
Co-stimulatory and Inhibitory Receptors | ||
CD159a (NKG2A) | ||
PD-1 | ||
TIGIT | ||
CD357 (GITR) | ||
TIM-3 | ||
CD226 (DNAM1) | ||
Proliferation markers | ||
Ki-67 | Proliferating cells | |
Cytokines | ||
IFN-gamma | Typical TH1 cytokine | |
Live/Dead | ||
Live/Dead | Allows identification of dead cells |
CD4 T cell & Treg activation and phenotyping
Panel focus: | Deep dive into CD4 T cell and Treg subpopulations, exhaustion status and ex vivo activation marker upregulation |
Populations markers | ||
CD8 / CD14 / CD20 | Exclusion markers (Dump) | |
CD4 | T helper cells | |
Differentiation- /Subpopulation markers | ||
CCR7 | Naive T cells | |
CD45RO | Memory T cells | |
Intb-7 | Integrin beta7 / Gut homing marker | |
CLA | Skin homing marker | |
CD39 | NTPDase1, hydrolyzes ATP+ADP / Regulatory T cells | |
CD127 | IL-7Ra (low expression on Treg) | |
FoxP3 | Treg transcription factor | |
Helios | Transcription factor on Treg population | |
Activation markers | ||
CD154 (CD40L) | Activated T helper cells | |
CD137 | Activated Treg | |
CD25 | Activated T cells and regulatory cells (Treg) | |
CD38 | Activated T cells | |
Co-stimulatory and Inhibitory Receptors | ||
PD-1 | ||
TIGIT | ||
CTLA-4 | ||
Proliferation markers | ||
Ki67 | Cell division marker | |
Cytokines | ||
IL-17 | Typical TH17 cytokine | |
IFN-g | Typical TH1 cytokine | |
TNF-a | ||
IL-2 | ||
IL-10 | Immune regulatory cytokine | |
IL-4 | Typical TH2 cytokine | |
Live/Dead | ||
Live/Dead | Allows identification of dead cells |
Cyto+ Panel
The Cyto+ panel is a customizable standard panel with slots reserved for interchangeable markers. A standardized bioinformatic analysis pipeline is also available for this panel.
| Population Markers (Major leukocyte lineages) | ||
| CD45 | All leukocytes (pan‑leukocyte) | |
| CD3 | T cells | |
| CD4 | CD4 T cells (T helper) | |
| CD8 | CD8 T cells (cytotoxic T cells) | |
| TCRγδ (TCRgd) | γδ T cells | |
| CD19 | B cells (pan‑B) | |
| CD20 | Mature B cells (usually not plasmablasts) | |
| IgD | Naive B cells (mature naive) | |
| IgM | Naive/unswitched B cells | |
| CD14 | Monocytes (especially classical CD14++) | |
| CD33 | Myeloid cells (mono/DC) | |
| CD11b | Myeloid cells (mono/granulocytes) | |
| CD11c | Monocytes + myeloid DC (cDC) | |
| HLA‑DR | APCs (B cells, monocytes, DC) | |
| CD1c | cDC2 (BDCA‑1 DC) | |
| CD123 | pDC (high) / also basophils | |
| CD15 | Granulocytes (esp. neutrophils) | |
| CD66 (usually CD66b) | Neutrophils (very typical granulocyte marker) | |
| CD56 | NK cells (CD3−CD56+) | |
| Differentiation / Subpopulation Markers | ||
| CD45RA | Naive T cells (vs memory) | |
| CD27 | Memory B cells (CD27+) / also T‑cell memory status | |
| CD28 | T‑cell differentiation (CD28+ early; CD28− more effector/late) | |
| CD57 | Terminally differentiated NK/T (e.g., CD8+CD57+) | |
| CD16 | NK subset (CD56dimCD16hi) / monocyte subsets (CD16+) / neutrophils | |
| CD5 | T cells + CD5+ B‑cell subset (T‑like B) | |
| CD161 | MAIT/innate‑like T (often CD161high) & NK subsets | |
| CD127 (IL‑7Rα) | Treg discrimination (Tregs tend to be CD127low) / memory T often high | |
| Activation Markers | ||
| CD25 | Tregs (CD4+CD25high) / activated T cells | |
| CD38 | Plasmablasts (CD38high) / activated T/B | |
| HLA‑DR (T cells) | Activated T cells (when strongly expressed) | |
| Trafficking / Homing (Chemokine receptors) | ||
| CCR7 | Naive + central memory T (lymph‑node homing) | |
| CCR5 | Inflammatory effector T / tissue migration | |
| CCR6 (CD196) | Th17‑like CD4 T (often CCR6+) | |
| CCR4 | Th2‑like / Treg‑adjacent profiles | |
| CXCR3 (CD183) | Th1‑like / effector T (inflammatory) | |
| CXCR5 (CD185) | Tfh (CD4+CXCR5+) & follicular homing | |
| Open channels (can not be used all simultaneously) | BV421 | |
| BV650 | ||
| FITC | ||
| PE | ||
| PE-CY7 | ||
| APC | ||
| APC-CY7 |
Custom Panel Design
For specific research questions, we offer fully customized panel design as part of a scientific collaboration. Please contact us to discuss your experimental needs.
Operator-assisted Flow Cytometry
- Cell Sorting
We generally offer cell sorting only as a full-service operator-assisted option to ensure highest purity and yield. For projects requiring frequent sorting with less complex setups, we offer training for the BD Melody Cell Sorter.
- Service measurements
We offer a modular System for operator-assisted service options. Prices depend on panel complexity and workload.
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- Full Service: Includes materials, instrument time, and operator hours (suitable for smaller sample sizes).
- Collaborative Service: For larger studies (clinical cohorts), reagents are typically provided by the user or total reagents are charged separately.
User-operated Flow Cytometry
- Instrument Training
Independent usage of our analyzers requires mandatory initial training to ensure data quality and instrument safety.
- Who: All users (regardless of prior experience).
- Content: General handling, facility safety, and specific Quality Assurance (QA) protocols.
- Booking: Please contact us to schedule your introduction.
Data Analysis & Bioinformatics
- We support you in the analysis of your flow cytometry data. Please contact us so we can help you finding the right solution.
- Need help interpreting your high-dimensional data? Our CYTO+ unit specializes in AI-driven clustering, dimensionality reduction (UMAP/tSNE), and automated pipeline analysis.
Find out about CYTO+ AI-Powered Analysis.