Panel design
We support your study with expert flow cytometry panel design. Whether you need a completely custom setup or an adaptation of established panels, we find the right solution for your research question.
Available Flow Cytometry Panels
For straightforward measurements, we can offer in-house validated panels or validated published standard panels.
T cell and innate Subset phenotyping
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Panel focus: |
Lymphocyte immunophenotyping with focus on T cells and small innate and innate-like populations |
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Populations markers |
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CD1c |
Dendritic cells |
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CD3 |
T cells |
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TCR V alpha 7.2 |
MAIT cells (invariant abTCR) |
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TCRVdelta1 |
T cells (Vd1 TCR) |
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TCRVdelta2 |
T cells (Vd1 TCR) |
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TCRgamma/delta |
T cells (pan-gd TCR) |
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CD4 |
CD4+ T helper cells |
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CD8 |
CD8+ Cytotoxic T cells |
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CD14 |
Monocytes |
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CD16 (FcγRIII) |
NK cells, monocytes, macrophages, neutrophil granulocytes, certain T cells |
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CD19 |
B cells |
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CD33 |
Myeloid linage |
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CD45 |
Leukocytes |
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CD56 |
NK and NK T cells |
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CD64 |
Monocytes, Macrophages |
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Differentiation- /Subpopulation markers |
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CD25 |
Activated T cells and regulatory cells (Treg) |
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CD27 |
Central memory- & naïve T cells |
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CD45RA |
Terminal differentiated- & naive T cells |
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CD117 (cKit) |
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CD127 |
IL7Ra (low expression on Treg) |
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CD197 (CCR7) |
Naive T cells |
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CD294 CRTH-2 |
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Activation markers |
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CD38 |
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HLA-DR |
MHC class II molecule |
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Co-stimulatory and Inhibitory Receptors |
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CD159a (NKG2A) |
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CD314 (NKG2D) |
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CD337 (NKp30) |
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CD335 (NKp46) |
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CD161 (KLRG1) |
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Live/Dead |
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Live/Dead |
Allows identification of dead cells |
T- & NK cell activation and phenotyping
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Panel focus:
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Identification of subpopulations and exhaustion status, and ex vivo activation marker upregulation |
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Populations markers |
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CD3 |
T cells |
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TCRVdelta1 |
T cells (Vd1 TCR) |
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TCRVdelta2 |
T cells (Vd1 TCR) |
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TCRgamma/delta |
T cells (pan-gd TCR) |
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CD4 |
CD4+ T helper cells |
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CD8 |
CD8+ Cytotoxic T cells |
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CD16 (FcγRIII) |
NK cells, monocytes, macrophages, neutrophil granulocytes, certain T cells |
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CD56 |
NK and NK T cells |
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Differentiation- /Subpopulation markers |
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CCR7 |
Naive T cells |
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CD27 |
Central memory- & naïve T cells |
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CD45RA |
Terminal differentiated- & naive T cells |
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CD25 |
Activated T cells and regulatory cells (Treg) |
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CD127 |
IL7R (low expression on Treg) |
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FoxP3 |
Treg cells |
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Activation markers |
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CD25 |
Activated T cells and regulatory cells (Treg) |
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CD38 |
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CD69 |
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CD137 |
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CD154 (CD40L) |
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HLA-DR |
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Co-stimulatory and Inhibitory Receptors |
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CD159a (NKG2A) |
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PD-1 |
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TIGIT |
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CD357 (GITR) |
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TIM-3 |
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CD226 (DNAM1) |
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Proliferation markers |
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Ki-67 |
Proliferating cells |
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Cytokines |
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IFN-gamma |
Typical TH1 cytokine |
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Live/Dead |
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Live/Dead |
Allows identification of dead cells |
CD4 T cell & Treg activation and phenotyping
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Panel focus: |
Deep dive into CD4 T cell and Treg subpopulations, exhaustion status and ex vivo activation marker upregulation |
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Populations markers |
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CD8 / CD14 / CD20 |
Exclusion markers (Dump) |
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CD4 |
T helper cells | |
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Differentiation- /Subpopulation markers |
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CCR7 |
Naive T cells |
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CD45RO |
Memory T cells |
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Intb-7 |
Integrin beta7 / Gut homing marker |
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CLA |
Skin homing marker |
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CD39 |
NTPDase1, hydrolyzes ATP+ADP / Regulatory T cells |
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CD127 |
IL-7Ra (low expression on Treg) |
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FoxP3 |
Treg transcription factor |
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Helios |
Transcription factor on Treg population |
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Activation markers |
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CD154 (CD40L) |
Activated T helper cells |
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CD137 |
Activated Treg |
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CD25 |
Activated T cells and regulatory cells (Treg) |
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CD38 |
Activated T cells |
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Co-stimulatory and Inhibitory Receptors |
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PD-1 |
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TIGIT |
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CTLA-4 |
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Proliferation markers |
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Ki67 |
Cell division marker |
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Cytokines |
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IL-17 |
Typical TH17 cytokine |
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IFN-g |
Typical TH1 cytokine |
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TNF-a |
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IL-2 |
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IL-10 |
Immune regulatory cytokine |
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IL-4 |
Typical TH2 cytokine |
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Live/Dead |
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Live/Dead |
Allows identification of dead cells |
Cyto+ Panel
The Cyto+ panel is a customizable standard panel with slots reserved for interchangeable markers. A standardized bioinformatic analysis pipeline is also available for this panel.
| Population Markers (Major leukocyte lineages) | ||
| CD45 | All leukocytes (pan‑leukocyte) | |
| CD3 | T cells | |
| CD4 | CD4 T cells (T helper) | |
| CD8 | CD8 T cells (cytotoxic T cells) | |
| TCRγδ (TCRgd) | γδ T cells | |
| CD19 | B cells (pan‑B) | |
| CD20 | Mature B cells (usually not plasmablasts) | |
| IgD | Naive B cells (mature naive) | |
| IgM | Naive/unswitched B cells | |
| CD14 | Monocytes (especially classical CD14++) | |
| CD33 | Myeloid cells (mono/DC) | |
| CD11b | Myeloid cells (mono/granulocytes) | |
| CD11c | Monocytes + myeloid DC (cDC) | |
| HLA‑DR | APCs (B cells, monocytes, DC) | |
| CD1c | cDC2 (BDCA‑1 DC) | |
| CD123 | pDC (high) / also basophils | |
| CD15 | Granulocytes (esp. neutrophils) | |
| CD66 (usually CD66b) | Neutrophils (very typical granulocyte marker) | |
| CD56 | NK cells (CD3−CD56+) | |
| Differentiation / Subpopulation Markers | ||
| CD45RA | Naive T cells (vs memory) | |
| CD27 | Memory B cells (CD27+) / also T‑cell memory status | |
| CD28 | T‑cell differentiation (CD28+ early; CD28− more effector/late) | |
| CD57 | Terminally differentiated NK/T (e.g., CD8+CD57+) | |
| CD16 | NK subset (CD56dimCD16hi) / monocyte subsets (CD16+) / neutrophils | |
| CD5 | T cells + CD5+ B‑cell subset (T‑like B) | |
| CD161 | MAIT/innate‑like T (often CD161high) & NK subsets | |
| CD127 (IL‑7Rα) | Treg discrimination (Tregs tend to be CD127low) / memory T often high | |
| Activation Markers | ||
| CD25 | Tregs (CD4+CD25high) / activated T cells | |
| CD38 | Plasmablasts (CD38high) / activated T/B | |
| HLA‑DR (T cells) | Activated T cells (when strongly expressed) | |
| Trafficking / Homing (Chemokine receptors) | ||
| CCR7 | Naive + central memory T (lymph‑node homing) | |
| CCR5 | Inflammatory effector T / tissue migration | |
| CCR6 (CD196) | Th17‑like CD4 T (often CCR6+) | |
| CCR4 | Th2‑like / Treg‑adjacent profiles | |
| CXCR3 (CD183) | Th1‑like / effector T (inflammatory) | |
| CXCR5 (CD185) | Tfh (CD4+CXCR5+) & follicular homing | |
| Open channels (can not be used all simultaneously) | BV421 | |
| BV650 | ||
| FITC | ||
| PE | ||
| PE-CY7 | ||
| APC | ||
| APC-CY7 |
Custom Panel Design
For specific research questions, we offer fully customized panel design as part of a scientific collaboration. Please contact us to discuss your experimental needs.
Operator-assisted Flow Cytometry
- Cell Sorting
We generally offer cell sorting only as a full-service operator-assisted option to ensure highest purity and yield. For projects requiring frequent sorting with less complex setups, we offer training for the BD Melody Cell Sorter.
- Service measurements
We offer a modular System for operator-assisted service options. Prices depend on panel complexity and workload.
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- Full Service: Includes materials, instrument time, and operator hours (suitable for smaller sample sizes).
- Collaborative Service: For larger studies (clinical cohorts), reagents are typically provided by the user or total reagents are charged separately.
User-operated Flow Cytometry
- Instrument Training
Independent usage of our analyzers requires mandatory initial training to ensure data quality and instrument safety.
- Who: All users (regardless of prior experience).
- Content: General handling, facility safety, and specific Quality Assurance (QA) protocols.
- Booking: Please contact us to schedule your introduction.
Data Analysis & Bioinformatics
- We support you in the analysis of your flow cytometry data. Please contact us so we can help you finding the right solution.
- Need help interpreting your high-dimensional data? Our CYTO+ unit specializes in AI-driven clustering, dimensionality reduction (UMAP/tSNE), and automated pipeline analysis.
Find out about CYTO+ AI-Powered Analysis.